An homologous radioimmunoassay for coho salmon (Oncorhynchus kisutch) vitellogenin, with general applicability to other Pacific salmonids.
Benfey TJ, Donaldson EM, Owen TG.
Department of Fisheries and Oceans, West Vancouver Laboratory, British Columbia, Canada.
This paper describes an homologous radioimmunoassay for coho salmon vitellogenin that demonstrates parallel cross-reactivity for plasma vitellogenin of all Pacific salmonids tested (chinook, chum, coho, pink, and sockeye salmon, and cutthroat and rainbow trout), but not for Atlantic salmon or two nonsalmonids: common carp and sablefish. Plasma vitellogenin levels were high in ovulatory female Pacific salmonids (micrograms/ml to mg/ml range), but were mostly nondetectable in spermiating males of the same species.
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PMID: 2767411 [PubMed - indexed for MEDLINE]