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Lactate and glucose measurements in supernatants obtained from dishes with or without cells. |
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| Culture Supernatants |
Time (h) |
Lactate (mmol/L/106 cells) |
Glucose (mmol/L/106 cells) |
|
|
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| Cell-free (20% O2) |
0–24 |
0.10 (± 0.20) |
11.81 (± 0.24) |
| LSECs (20% O2) |
0–24 |
1.19 (± 0.42) |
10.96 (± 0.40) |
| LSECs (5% O2) |
0–24 |
2.77 (± 0.59) |
10.83 (± 0.20) |
| LSECs (20% O2) |
24–48 |
1.31 (± 0.38) |
10.70 (± 0.20) |
| LSECs (5% O2) |
24–48 |
3.07 (± 1.12) |
10.36 (± 0.20) |
|
Conditioned media collected from cell free incubations, or LSEC cultures were analysed for glucose and lactate. Values are means of triplicate measurements. The results are representative data obtained from three independent experiments. | |||
Martinez et al. Comparative Hepatology 2008 7:4 doi:10.1186/1476-5926-7-4 |
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