Figure 1.

Morphological examination of LSEC cultures over time by light microscopy. Freshly isolated LSECs cultures were established on 24 well plates and incubated either at hyperoxia (a-c) or at normoxia (d-f). The general morphology of the cultures was monitored by light microscopy at day 1 (a, d), day 3 (b, d) and day 5 (c, f) after isolation. Decline of LSECs cultures may be observed in dishes maintained at atmospheric oxygen levels (a-c) after several days of culture.