|
Synopsis of the predicted TGF-β and GR response elements in PAI-1 and CTGF promoter sequences |
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| Gene |
Promoter motifs |
Pathway |
Promoter sequence |
Ref. |
|
|
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| hCTGF |
GTGTCAAGGGGTCAGGAT |
TGF-β inducible |
-162 to 128 |
[14] |
| hCTGF |
GAGGAATGCGAGGAATG |
TGF-β-Smad3/4 |
-805 to +17 |
[15] |
| hCTGF |
- |
GR |
-292 to +22 |
[25] |
| mCTGF |
No direct binding, interaction with other TF (STAT3?) |
GR |
-897 to -628 (mouse strain specific) |
[19] |
| hPAI-1 |
AG(C/A)CAGACA |
TGF-β-Smad3/4 |
-730, -580, -280 |
[26] |
| hPAI-1 |
AG(C/A)CAGACA |
TGF-β-Smad3/4 |
-732 to -721 |
[27] |
| PAI-1 |
MLP(CAGA)9-luc |
TGF-β-Smad3/4 |
Reporter plasmid |
[28] |
| hPAI-1 |
- |
GR |
-800, -549, -100, +75, |
[17] |
| rPAI-1 |
AGCACACACTGTTCT |
GR |
-1212 to -1196 |
[29] |
|
Besides the transcriptional activation of gene expression through potentially response elements of single transcription factors, and apart from a cell-type specific composition of those transcription factors, a superior regulation is conceivable, e.g. a diversity in methylation sites or the condensation status of the chromatin. GTCTAGAC: TGF-β-Smad3/4 consensus sequence; GGTACANNNTGTTCT: GR consensus sequence; r: rat; h: human; m: mouse. | ||||
Wickert et al. Comparative Hepatology 2007 6:5 doi:10.1186/1476-5926-6-5 |
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