Figure 5.

Expression of AFP gene and Rev-Erb A, Rev-Erb B and N-CoR in the rat model of acute liver damage. After CCl₄ treatment, the expression of the AFP gene (A) and of Rev-Erb A, Rev-Erb B and N-CoR (B) were measured by real-time PCR. The mean values and the standard deviations of two different series are shown. A decrease of full-length AFP-RNA (AFP1; – 4.5 fold) and the smaller splicing products (AFP2; -4.5 fold) could be seen after 12 hours; afterwards, a continuous increase could be observed of both products with a maximum after 72 hours (AFP1 up to 7.0 fold and AFP2 up to 4.8 fold). A continuous down regulation of Rev-Erb A with the maximum at 48 hours could be observed, 24 hour later both AFP products were maximally up regulated. Rev-Erb and N-CoR show only slight changes in this model.