Figure 4.

Expression of AFP gene and Rev-Erb A, Rev-Erb B and N-CoR in liver regeneration without 2-AAF treatment. After partial hepatectomy without 2-AAF treatment the expression of AFP gene (A) and of Rev-Erb A, Rev-Erb B and N-CoR (B) were measured by real-time PCR, in the rat liver. The mean values and the standard deviations of two different series are demonstrated. From 2 up to 16 hours, a down regulation of the smaller variants of AFP-RNA (AFP2) could be seen. Compared to 16 hours value an increase of the smaller splicing products up to -5.1 fold could be observed after 24 hours; afterwards, the level do not change up to 72 hours. The full-length AFP-RNA shows only slight changes. The genes of orphan nuclear receptors are down regulated at each time point. Rev-Erb A and Rev-Erb B are maximally down regulated after 16 hours similarly to the smaller variants of AFP-RNA (AFP2).