Figure 1.
Expression of Ob-R mRNA in sinusoidal cells.Hepatic stellate cells (HSCs), sinusoidal endothelial cells (SECs), and Kupffer cells were isolated from Wistar rats, and cultured for 7 days, 3 days and 1 day, respectively. Total RNA (1 microgram) from each cell type was analyzed by RT-PCR for Ob-Ra, Ob-Rb, and beta-actin as described in Methods.
Ikejima et al. Comparative Hepatology 2004 3(Suppl 1):S12 doi:10.1186/1476-5926-2-S1-S12