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Plasma levels of investigated parameters of Sprague-Dawley rats after in vivo treatment with nitric oxide donor (SNP) and peroxynitrite. Data expressed as Mean (SD); n = 8 per group. |
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| Parameters |
Control |
SNP |
Peroxynitrite |
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| Lipid peroxidation (MDA) (μmol/l) |
7.10 (0.86) |
6.78 (2.11) |
6.64 (2.85) |
| SH groups (μmol/l) |
180.47 (36.08) |
150.84 (4.78) |
110.40 (11.96)* |
| NO2+NO3 (μmol/l) |
45.47 (18.80) |
66.82 (4.12) |
81.94 (10.18) |
| Uric acid (μmol/l) |
64.82 (4.18) |
22.34 (8.32) |
17.50 (3.21)* |
| Carbonyl groups (μmol/g protein) |
179.59 (28.50) |
144.90 (67.40) |
169.06 (14.88) |
| Nitrotyrosine (nmol/g protein) |
96.02 (26.53) |
136.17 (27.47) |
135.11 (4.64) |
| ALT (U/l) |
31.9 (4.15) |
34.44 (4.96) |
28.5 (5.15) |
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Male Sprague-Dawley rats three months old were allocated into three different groups. Either peroxynitrite (0.5 ml/kg BW of 30 mmol solution) or SNP (10 mg/kg BW) in a volume of 100 μl were administrated in bolus in systemic circulation. The control group received physiological saline solution at the same volume. All procedures were carried out as described in Methods. Asterisk: significantly different from the control (p < 0.05). | |||
Kocic et al. Comparative Hepatology 2004 3:6 doi:10.1186/1476-5926-3-6 |
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