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Specific activities of lysosomal enzymes in parenchymal (PC), sinusoidal endothelial cells (LSEC) and Kupffer cells (KC) isolated from pig liver. |
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| LSEC |
KC |
PC |
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| α-Mannosidase (= 7) |
0.76a (0.14) |
0.59b (0,16) |
0.40c (0.08) |
| Hexosaminidase (n = 7) |
30.3a (7.86) |
29.2a (1.70) |
4.02b (2.06) |
| Acid lipase (n = 6) |
6.66a (2.40) |
4.33ab (2.60) |
2.03b (0.46) |
| Acid phosphatase (n = 6) |
11.2a (3.90) |
8.89ab (2.72) |
4.97b (1.96) |
| Glucuronidase (n = 5) |
3.55a (0.77) |
2.35b (0.53) |
0.52c (0.28) |
| Aryl sulphatase (n = 4) |
0.27a (0.11) |
0.20a (0.05) |
0.05b (0.02) |
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Each experiment consisted of three parallels. Values are means (SD). The letters (a, b, and c) indicate significant statistical differences between the values of the different cell types. Enzyme activities are expressed as 106 4-methylumbelliferyl (-mannopyranoside, -N-acetyl-glucosaminide, -oleate, -phosphate, -glucuronide, -sulfate) molecules released per min per g cell solubilisate at 37°C. (p < 0.05, ANOVA, LSD post hoc test). | |||
Elvevold et al. Comparative Hepatology 2004 3:4 doi:10.1186/1476-5926-3-4 |
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