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Evaluation of apoptosis versus necrosis in HepG2 cultures incubated with 10 mM Pb(NO3)2, Pb(C2H3O3)2 or KNO3 and with conditioned medium from Kupffer cells incubated with10 mM Pb(NO3)2 for 24 hours.* |
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| Treatments |
6 hours |
8 hours |
16 hours |
24 hours |
48 hours |
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|
|
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| Necrosis |
Apoptosis |
Necrosis |
Apoptosis |
Necrosis |
Apoptosis |
Necrosis |
Apoptosis |
Necrosis |
Apoptosis |
|
|
|
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| Control |
2.00 |
0.80 |
2.05 |
0.75 |
2.50 |
1.20 |
2.70 |
3.51 |
3.06 |
4.01 |
| Pb(NO3)2 |
1.80 |
0.90 |
2.05 |
1.17 |
2.84 |
1.15 |
13.73^ |
4.35 |
25.00^ |
9.20 |
| Pb(C2H3O2)2 |
4.50^ |
2.10 |
18.90^ |
1.80 |
35.30^ |
3.10 |
81.03^ |
7.50^ |
92.60^ |
15.20^ |
| KNO3 |
1.42 |
0.18 |
1.20 |
0.53 |
2.69 |
0.70 |
7.71 |
1.19 |
15.30^ |
12.58^ |
| C.M. 1 |
0.80 |
1.10 |
2.10 |
1.50 |
2.50 |
2.00 |
3.80 |
3.20 |
4.10 |
3.80 |
| C.M. 2 |
1.70 |
3.10^ |
3.20 |
4.50^ |
2.80 |
10.10^ |
3.20 |
18.50^ |
3.80 |
25.30^ |
| C.M. 3 |
0.90 |
2.00 |
1.80 |
3.10 |
1.90 |
2.30 |
2.50 |
7.10 |
2.90 |
4.20 |
|
* Control animals were injected with saline. Apoptosis and necrosis were detected on slides of haematoxylin-eosin stained cells counting at least 150 cells in at least 10 randomly selected fields. C.M. 1 = conditioned medium collected from normal untreated Kupffer cell cultures. C.M. 2 = conditioned medium collected from Kupffer cell cultures incubated for 24 hours with Pb(NO3)2 (10 mM). C.M. 3 = conditioned medium collected from Kupffer cell cultures incubated for 24 h with KNO3 (10 mM). Data are given as percentages. Standard deviations did not exceed 5%. ^Significantly different in relation to Control (p < 0.05). | ||||||||||
Pagliara et al. Comparative Hepatology 2003 2:8 doi:10.1186/1476-5926-2-8 |
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