|
Evaluation of apoptosis versus necrosis in hepatocyte cultures incubated with 10 mM Pb(NO3)2, Pb(C2H3O2)2 or KNO3 and with CM from Kupffer cells.* |
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| Treatments |
Hours |
||||
|
|
|||||
| 6 |
8 |
16 |
24 |
48 |
|
|
|
|||||
| Control |
- |
- |
- |
± |
± |
| Pb(NO3)2 |
- |
- |
- |
± |
+++ |
| Pb(C2H3O2)2 |
- |
- |
± |
+++ |
++++ |
| KNO3 |
- |
- |
- |
± |
± |
| C.M. |
- |
- |
- |
Apoptosis (35%) |
Apoptosis (48%) |
|
* Control animals were injected with saline. The presence of necrosis is represented by +: ± <20%; + 20–40%; ++ 40–60%; +++ 60–80%; ++++ >80%. C.M. = conditioned medium collected from Kupffer cell cultures incubated for 24 h with Pb(NO3)2 (10 mM). Apoptosis was detected on slides of haematoxylin-eosin stained cells, counting at least 300 cells in at least 10 randomly selected fields. | |||||
Pagliara et al. Comparative Hepatology 2003 2:8 doi:10.1186/1476-5926-2-8 |
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