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Time-course of necrosis, apoptosis and DNA synthesis in hepatocytes isolated from control, Pb(NO3)2-treated, and co-administered GdCl3 plus Pb(NO3)2 rats.* |
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| Control |
Pb(NO3)2 |
GdCl3 + Pb(NO3)2 (2 hours) |
GdCl3 + Pb(NO3)2 (4 hours) |
GdCl3 + Pb(NO3)2 (24 hours) |
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| % |
1 day |
3 days |
5 days |
1 day |
3 days |
5 days |
1 day |
3 days |
5 days |
1 day |
3 days |
5 days |
|
|
|
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| Necrosis |
5 (1.2) |
7 (2.1) |
8 (2.1) |
5 (1.6) |
15^(2.7) |
16^(4.2) |
20 (3.5) |
13^(2.1) |
15^(2.9) |
21^(4.1) |
15^(4.3) |
12^(3.8) |
10^(1.8) |
| Apoptosis |
1 (0.2) |
1 (0.2) |
17^(5.3) |
45^(8.0) |
2 (0.9) |
15^(2.3) |
40 (4.1) |
3 (0.5) |
14^(3.5) |
33^(6.1) |
2 (0.9) |
8^(2.6) |
20^(3.4) |
| DNA** |
4 (1.0) |
3 (0.7) |
10^(3.4) |
9 (3.1) |
5 (1.3) |
12^(3.2) |
6 (2.2) |
6 (0.9) |
12^(3.6) |
7 (3.1) |
1 (0.2) |
8 (2.1) |
5 (0.9) |
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* Values are the mean (standard deviation) of at least three independent experiments of flow cytometry analysis. For each value at least 10 000 events were counted. ^Significantly different in relation to Control (p < 0.05). ** % of cells synthesizing DNA | |||||||||||||
Pagliara et al. Comparative Hepatology 2003 2:8 doi:10.1186/1476-5926-2-8 |
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