|
Identification of cells after cultivation of elutriation fractions. |
||||
| Fraction |
FITC – FSA |
Desmin |
KC |
Hepatocytes |
|
|
||||
| 1 |
32.1 (12.6) |
63.9 (15.4) |
4.0 (6.9) |
0.0 (0.0) |
| 2 |
80.4 (6.4) |
10.9 (9.2) |
7.0 (1.4) |
1.7 (1.6) |
| 3 |
66.2 (11.1) |
7.1 (3.8) |
15.2 (13.5) |
11.5 (17.5) |
| 4 |
61.0 (17.5) |
2.1 (1.7) |
10.9 (10.9) |
26.0 (19.4) |
|
Prior to isolation of cells, pigs received FITC-labeled formaldehyde-treated serum albumin (FSA) intravenously. Stellate cells stained with monoclonal mouse anti-human desmin antibody and Kupffer cells (KC) stained with anti-pig macrophage antibodies. Hepatocytes were identified by simple morphology. Values are percent of total number of cells per culture (n = 3). The values are expressed as: mean (standard deviation). | ||||
Nedredal et al. Comparative Hepatology 2003 2:1 doi:10.1186/1476-5926-2-1 |
||||